Marine bioactives from Saccharina latissima

Inflammatory bowel disease (IBD) is a term used to describe a group of inflammatory conditions which includes Crohn's disease and ulcerative colitis. The use of seaweed and seaweed extracts in the treatment of inflammatory conditions of the gastrointestinal tract has generated increased interest due to their potent immunomodulatory and anti-inflammatory potential. Therefore an in vitro model of gastrointestinal inflammation is required to assess the anti-inflammatory potential of marine bioactives from brown seawwed (see figure). After an extensive literature search, a co-culture system using the intestinel epithelial cell line CaCo-2 and the murine macrophage cell line RAW264.7 was chosen to simulate gastrointestinal inflammation. Thsi co-culture system will be treated with brown macroalgae extracts and stimulated with lipopolysaccharide. The production of relevant pro-inflammatory cytokines, such as IL-8 and TNF-alpha, will be assessed through ELISA essays or through quantitative PCR.